NanoTemper’s mission is to enable everyone to do science that matters by always pushing the limits. NanoTemper was started in 2008 after Stefan and Philipp met at Ludwig-Maximilians-University Munich. At the time, the effect of thermophoresis was not known within the Life Sciences community, but Stefan and Philipp knew that it was important. Realizing the shortcomings of current protein research techniques, they came up with a method, MicroScale Thermophoresis that was enormously sensitive, and would bring researchers one step closer to developing better drugs faster.
Now, NanoTemper has over 150 employees with representation in 13 countries around the world including Germany, UK, Poland, Denmark, Belgium, Netherlands, Spain, France, USA, Brazil, Russia, China, and India and also a number of exclusive partners/distributors worldwide.
Ansar-analitika d.o.o. as an exclusive representative of NanoTemper in the Republic of Croatia, offers sales and after-sales support, as well as service for NanoTemper devices.
NanoTemper products:
Affinity screening
Dianthus
Dianthus is a plate-based, microfluidics-free screening platform that requires no surface immobilization, and measures under controlled equilibrium conditions – perfect for challenging affinity screenings. And not only that, Dianthus is a great choice if you’re looking for a complementary or orthogonal technique to validate hits and leads identified by other biophysical methods.
Binding affinity
Monolith
Monolith measures the broadest range of binding affinities in-solution, using very little sample.
Knowing the strength of the interactions between key players will give you the insights you need to understand the details behind how biological systems work. If you’re having difficulty studying challenging interactions that include membrane proteins, PROTACs, intrinsically disordered proteins (IDPs), or RNA-based therapeutics, you’ll need Monolith to characterize them.
Advantages of Monolith:
- With a broad sensitivity range, Monolith measures Kds varying from pM to mM, so you can tackle as many projects as you want
- Move forward with the best candidates quickly—get accurate Kd values in minutes using very
- little sample consumption
- Characterize interactions with many different types of molecules or samples: e.g. proteins (membrane proteins, intrinsically disordered proteins (IDPs), receptors, enzymes, antibodies, and nanobodies), peptides, small molecules (fragments, PROTACs, ions, nanoparticles, and carbohydrates), Nucleic acids (DNA, RNA, and aptamers), platelets and whole cells, virus particles and empty capsids Koristite vrlo malo uzorka – 3 µL do 4 µL
- Analyze samples in any buffer or bioliquid and in close-to-native conditions without purification or immobilization steps
- Optimize assays easily
As interactions are characterized free in solution, MST has been successful where SPR failed due to the limitations of a surface-based approach.
Dr. Stephan Uebel
Max-Planck-Institute of Biochemistry, Germany
Protein stability + aggregation + particle sizing
Prometheus
Prometheus has been chosen by the scientific community because it consistently delivers high-resolution data that reveals liabilities in drug candidates missed by other technologies. No other system offers simultaneous, multi-parameter characterization throughout the entire run — measuring thermal unfolding, particle sizing, and aggregation — as well as chemical denaturation for a stability profile of unmatched detail.
Prometheus utilizes a number of technologies to characterize thermal unfolding, particle sizing, and aggregation. Each one tells a different story about stability, and is used for a variety of applications. But they have one thing in common — they are all label-free.
Protein quality
Tycho
Tycho tells you so much about the quality of your protein
It tells you about presence, purity, concentration, functionality and similarity — in a single experiment. These can all be measured simply by determining whether your protein is structurally intact or properly folded.
- Analyze under native conditions
- Get answers in minutes
- Easily test any protein sample type
- Save scarce bench space
- Conserve precious sample
I put every protein in Tycho first. The data is very reproducible in our hands. Just mix your samples, put the capillaries in and 3 minutes later you have your results. It’s the only equipment I would recommend to anyone working on proteins because there are no downsides in having a Tycho.
Dr. Stefan Gajewski
Scientist I, Nurix Inc., California
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